Composite
Part:BBa_I20532:Design
Designed by: Jerome Bonnet Group: Endy Lab (2012-04-03)
Bxb1 BP Data Register
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 722
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1041
Illegal NheI site found at 1064
Illegal SpeI site found at 722 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1275
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 722
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 722
Illegal NgoMIV site found at 730 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 750
Illegal BsaI.rc site found at 1232
Illegal SapI.rc site found at 1313
Design Notes
Recombination sites can have some terminator activity-Strong RBS preferable at low copy number
Source
Multiple