Composite

Part:BBa_I20532:Design

Designed by: Jerome Bonnet   Group: Endy Lab   (2012-04-03)

Bxb1 BP Data Register


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 722
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1041
    Illegal NheI site found at 1064
    Illegal SpeI site found at 722
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1275
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 722
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 722
    Illegal NgoMIV site found at 730
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 750
    Illegal BsaI.rc site found at 1232
    Illegal SapI.rc site found at 1313


Design Notes

Recombination sites can have some terminator activity-Strong RBS preferable at low copy number

Source

Multiple

References